Tobacco Measures of Exposure Tobacco Biomarkers

Nicotine and Nicotine Related Metabolites:
Nicotine	Matrices – Serum, Plasma, Saliva, Urine
Cotinine	Matrices – Serum, Plasma, Saliva, Urine
Trans-3-Hydroxycotinine	Matrices – Saliva, Urine
Cotinine-N-b-D-glucuronide	Matrix – Urine
Nicotine-N-b-glucuronide	Matrix – Urine
trans-3-Hydroxycotinine-O-b-D-glucuronide	Matrix – Urine
Cotinine-N-Oxide	Matrix – Urine
Nicotine-1-Oxide	Matrix – Urine
Nornicotine	Matrix – Urine
Norcotinine	Matrix – Urine

Aromatic Amines:
1-Aminonaphtalene	Matrix – Urine
2-Aminonaphtalene	Matrix – Urine
3 and 4 Aminobiphenyl	Matrix – Urine
o-Toluidine	Matrix – Urine

Acrolein:
3-Hydroxypropyl mercapturic acid	Matrix – Urine

1,3 – butadiene:
Monohydroxybutenyl mercapturic acid	Matrix – Urine
Dihydroxylbutyl mercapturic acid	Matrix – Urine

Benzene:
S-Phenyl mercapturic Acid	Matrix – Urine

Caffeine	Matrices – Serum, Plasma, Saliva, Urine

Crotonaldehyde:
3-hydroxy-1methylpropylmercapturic acid	Matrix - Urine

Tobacco Specific Nitrosamines:
NAB and BAB-N-glucuronide*	Matrix - Urine
NAT and NAT-N-glucuronide*	Matrix - Urine
NNN and NNN-N-glucuronide*	Matrix - Urine
NNK and NNK-N-glucuronide*	Matrix - Urine
*Total TSNA and corresponding N-glucuronide

NNK:
NNAL and Conjugated glucuronides	Matrix - Urine

Polycyclic Aromatic Hydrocarbons:
1-Hydroxypyrene 1-Hydroxynaphthalene 2-Hydroxynaphthalene 2-Hydroxyfluorene 2-/3-hydroxyphenanthrene (sum of) 1-/9-hydroxyphenanthrene (sum of)	Matrix - Urine
Free Radicals:
8-hydroxy-2’-deoxyguanosine	Matrix - Urine

Urine Mutagenicity
Yield in Use (Filter Nicotine)